RNA

All posts tagged RNA

Clathrin & Primer Selection with the NCBI Database

Posted by DR. NURVUS on April 23, 2016
Posted in: Biochemistry. Tagged: , , , , , , , , , , , , , , , , , , . Leave a comment

A+K gel (edit)

Sometimes you need to find a primer. Not just any primer but a specific one. This is a small post concerning the development and acquisition of a specific primer for a portion of the clathrin protein. Clathrin is a protein that plays a key role in the process of endocytosis. It’s main function being to coat the inward bound lipid bilayer as it enters inwards into the cell. Its triskelion shape enables it to have the flexibility and reach needed for this specific function. Continue Reading

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A STUDY ON THE ISOLATION & ANALYSIS OF NUCLEIC ACIDS

Posted by DR. NURVUS on April 6, 2016
Posted in: Biochemistry. Tagged: , , , , , , , , , , , , , , . Leave a comment

cropped-dna-163466_1280.jpg

A Study on the Isolation & Analysis of Nucleic Acids

Basis in Isolation, Ultraviolet Spectroscopy, Restriction Endonuclease Digestions, Ligations, Hyperchromic Effect, Florescence Spectroscopy, and Agarose Gel Electrophoresis

 

Abstract

The quantitative analysis of nucleic acid samples can offer up insights into samples with unknown properties. In this brief study multiple techniques are utilized to isolate, quantify, and discern nucleic acid residues from Escherichia coli (E. coli) plasmids. Restriction digests and hyperchromic assays were also developed. Results for the restriction digest were not conclusive, but the hyperchromic effect was observed in both RNA and DNA as expected. The denaturant study with NaOH saw an interesting result, with RNA showing little change in spectroscopic absorbance, while DNA showed a drastic increase. Continue Reading