Sometimes you need to find a primer. Not just any primer but a specific one. This is a small post concerning the development and acquisition of a specific primer for a portion of the clathrin protein. Clathrin is a protein that plays a key role in the process of endocytosis. It’s main function being to coat the inward bound lipid bilayer as it enters inwards into the cell. Its triskelion shape enables it to have the flexibility and reach needed for this specific function. Continue Reading
As a scientist it is crucial to continue growing and learning as you progress in your field. That being the case, despite a lackluster background in the computer sciences I have come to rely more and more on computational skills in the lab. Ever so slowly the need for a stronger foundation in the tech fields has come to light and I feel woefully behind the times.
Computers are everywhere, whether you like it or not, and artificial intelligence, robotics, and coding are slowly taking over the world. Continue Reading
Analysis of Photosynthetic Membranes & Energy Production in Plants
Basis in Organelle Isolation, Photosynthesis Controls, and Membrane Energy Analysis with 2,4-Dinitrophenol Inhibitor
Photosynthesis is a vital chemical reaction for plants. The Hill reaction plays an important role in the overall scheme, transferring electrons, cleaving water, and consequently freeing oxygen. Effects of the inhibitor molecule 2,4-dinitrophenol (DNP) on multiple isolated chloroplast concentrations were assayed using a redox dye, 2,6-dichlorophenolindophenol (DCPIP). Suspected results proved true, with the expected outcomes showing the inhibitor to hinder photosynthetic reactions. The reduction of DCPIP was shown to cease throughout each sample where the inhibitor was added, showing a significant decrease in the reaction rate for chloroplasts.